In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus

Materials

Giovanna Bruni¹  Lorenzo Fassina²  Giulia Montagna³  Livia Visai³  Nora Bloise³  Rosalinda Caringella⁴  Alessia Patrucco⁴  Claudio Tonin⁴ 

[1] Center for Colloid and Surface Science (C.S.G.I.), Department of Chemistry, Section of Physical Chemistry, University of Pavia, Viale Taramelli, 16-27100 Pavia, Italy;Department of Electrical, Computer and Biomedical Engineering (DIII), Centre for Health Technologies (CHT), University of Pavia, Via Ferrata, 5-27100 Pavia, Italy;Department of Molecular Medicine (DMM), Centre for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli, 3/B-27100 Pavia, Italy;Institute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (STIIMA), Italian National Research Council (CNR), Corso Pella, 16-13900 Biella, Italy;

关键词: pulsed electromagnetic field;    osteogenic factors;    wool keratin scaffolds;    bone tissue engineering;   

DOI  :  10.3390/ma13143052

来源: DOAJ

【 摘 要 】

Pulsed electromagnetic field (PEMF) has drawn attention as a potential tool to improve the ability of bone biomaterials to integrate into the surrounding tissue. We investigated the effects of PEMF (frequency, 75 Hz; magnetic induction amplitude, 2 mT; pulse duration, 1.3 ms) on human osteoblast-like cells (SAOS-2) seeded onto wool keratin scaffolds in terms of proliferation, differentiation, and production of the calcified bone extracellular matrix. The wool keratin scaffold offered a 3D porous architecture for cell guesting and nutrient diffusion, suggesting its possible use as a filler to repair bone defects. Here, the combined approach of applying a daily PEMF exposure with additional osteogenic factors stimulated the cells to increase both the deposition of bone-related proteins and calcified matrix onto the wool keratin scaffolds. Also, the presence of SAOS-2 cells, or PEMF, or osteogenic factors did not influence the compression behavior or the resilience of keratin scaffolds in wet conditions. Besides, ageing tests revealed that wool keratin scaffolds were very stable and showed a lower degradation rate compared to commercial collagen sponges. It is for these reasons that this tissue engineering strategy, which improves the osteointegration properties of the wool keratin scaffold, may have a promising application for long term support of bone formation in vivo.

【 授权许可】

Unknown