期刊论文详细信息
Frontiers in Cell and Developmental Biology | |
Syndecan-4–/– Mice Have Smaller Muscle Fibers, Increased Akt/mTOR/S6K1 and Notch/HES-1 Pathways, and Alterations in Extracellular Matrix Components | |
Jan Magnus Aronsen1  Addolorata Pisconti2  Svein Olav Kolset3  Kristian Hovde Liland4  Cathrine Rein Carlson5  Marianne Lunde5  Ivar Sjaastad6  Geir Christensen6  Vibeke Høst7  Sissel Beate Rønning7  Mona Elisabeth Pedersen7  | |
[1]Bjørknes College, Oslo, Norway | |
[2]Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, United States | |
[3]Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway | |
[4]Faculty of Sciences and Technology, Norwegian University of Life Sciences, Ås, Norway | |
[5]Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway | |
[6]K.G. Jebsen Center for Cardiac Research, University of Oslo, Oslo, Norway | |
[7]Nofima AS, Ås, Norway | |
关键词: skeletal muscle; syndecan; exercise; myogenesis; Notch; decorin; | |
DOI : 10.3389/fcell.2020.00730 | |
来源: DOAJ |
【 摘 要 】
BackgroundExtracellular matrix (ECM) remodeling is essential for skeletal muscle development and adaption in response to environmental cues such as exercise and injury. The cell surface proteoglycan syndecan-4 has been reported to be essential for muscle differentiation, but few molecular mechanisms are known. Syndecan-4–/– mice are unable to regenerate damaged muscle, and display deficient satellite cell activation, proliferation, and differentiation. A reduced myofiber basal lamina has also been reported in syndecan-4–/– muscle, indicating possible defects in ECM production. To get a better understanding of the underlying molecular mechanisms, we have here investigated the effects of syndecan-4 genetic ablation on molecules involved in ECM remodeling and muscle growth, both under steady state conditions and in response to exercise.MethodsTibialis anterior (TA) muscles from sedentary and exercised syndecan-4–/– and WT mice were analyzed by immunohistochemistry, real-time PCR and western blotting.ResultsCompared to WT, we found that syndecan-4–/– mice had reduced body weight, reduced muscle weight, muscle fibers with a smaller cross-sectional area, and reduced expression of myogenic regulatory transcription factors. Sedentary syndecan-4–/– had also increased mRNA levels of syndecan-2, decorin, collagens, fibromodulin, biglycan, and LOX. Some of these latter ECM components were reduced at protein level, suggesting them to be more susceptible to degradation or less efficiently translated when syndecan-4 is absent. At the protein level, TRPC7 was reduced, whereas activation of the Akt/mTOR/S6K1 and Notch/HES-1 pathways were increased. Finally, although exercise induced upregulation of several of these components in WT, a further upregulation of these molecules was not observed in exercised syndecan-4–/– mice.ConclusionAltogether our data suggest an important role of syndecan-4 in muscle development.【 授权许可】
Unknown