Optimization of Enzyme-Assisted Extraction and Purification of Flavonoids from Pinus koraiensis Nut-Coated Film and Antioxidant Activity Evaluation

Molecules

Ximing Yang¹  Mingyan Zhang¹  Yuhang Lou¹  Xinxiu Xia¹  Hongyan Xu¹  Chao Wang²  Wuchao Ma³ 

[1] Department of Food Science and Engineering, Agricultural College, Yanbian University, Yanji 133000, China;Department of Food Science and Engineering, Fusion College, Yanbian University, Yanji 133000, China;Department of Food Science and Technology, Food College, Dalian Polytechnic University, Dalian 116000, China;

关键词: Pinus koraiensis nut-coated film;    flavonoids;    enzyme-assisted extraction;    response surface method;    macroporous resin;    antioxidant activity;   

DOI  :  10.3390/molecules26071950

来源: DOAJ

【 摘 要 】

Pinus koraiensis nut-coated film is a kind of by-product of nut processing, which has been shown to contain flavonoids, polyphenols, and other substances that can be used to produce natural antioxidant extracts. In this study, response surface methodology (RSM) was used to optimize the extraction process of flavonoids of P. koraiensis nut-coated film (PNF), and macroporous resin HPD600 was used to purify PNF (P-PNF). Its antioxidant activity was examined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging capacity, oxygen free radical absorption capacity (ORAC), total oxygen radical capture (TRAP), and iron ion reduction capacity. Under the ideal extraction conditions comprising a cellulase dosage of 90 U/g, a material/liquid ratio of 1:20 (g/mL), and an extraction time of 2 h, the PNF yield was 3.37%. Purification conditions were sample concentration of 2.0 mg/mL, pH of 5, water washing volume of 3 bed volume (BV), eluent ethanol concentration of 50%, and volume of 2 BV. The P-PNF recovery was 84.32%, and purity increased from 33.80% to 61.70%. Additionally, P-PNF showed increased antioxidant activity compared to PNF. Cumulatively, this study obtained the optimal values for the process parameters in order to achieve the maximum rates of extraction of PNF for economically optimal production at an industrial scale.

【 授权许可】

Unknown