Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy

International Journal of Molecular Sciences

Mayuki Ozawa¹  Nanami Ueda¹  Natsuko Kuroda¹  Maho Miyazaki¹  Haruna Terakado¹  Takuya Yoshizawa¹  Hiroyoshi Matsumura¹  Shun-ichi Tanaka¹  Takayuki Uchihashi²  Shogo Sugiyama²  Junso Fujita³ 

[1] Department of Biotechnology, College of Life Sciences, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga 525-8577, Japan;Department of Physics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan;Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan;

关键词: bacterial cell division;    Staphylococcus aureus;    FtsZ;    high-speed atomic force microscopy;   

DOI  :  10.3390/ijms22041697

来源: DOAJ

【 摘 要 】

FtsZ is a key protein in bacterial cell division and is assembled into filamentous architectures. FtsZ filaments are thought to regulate bacterial cell division and have been investigated using many types of imaging techniques such as atomic force microscopy (AFM), but the time scale of the method was too long to trace the filament formation process. Development of high-speed AFM enables us to achieve sub-second time resolution and visualize the formation and dissociation process of FtsZ filaments. The analysis of the growth and dissociation rates of the C-terminal truncated FtsZ (FtsZt) filaments indicate the net growth and dissociation of FtsZt filaments in the growth and dissociation conditions, respectively. We also analyzed the curvatures of the full-length FtsZ (FtsZf) and FtsZt filaments, and the comparative analysis indicated the straight-shape preference of the FtsZt filaments than those of FtsZf. These findings provide insights into the fundamental dynamic behavior of FtsZ protofilaments and bacterial cell division.

【 授权许可】

Unknown