Recent development of high-speed atomic force microscopy in molecular biology

Micro & nano letters

article

Ke Xu¹  Xin Huang¹  Yusheng Pan¹ 

[1] School of Information & Control Engineering, Shenyang Jianzhu University

关键词: molecular biophysics;    cantilevers;    atomic force microscopy;    macromolecules;    high-speed atomic force microscopy;    molecular biology;    biomacromolecule imaging;    HS-AFM;    biomacromolecule characteristics determination;    nanostructure recombination;    high-speed imaging;    cantilever;    mechanical scanner;    position sensor;    closed-loop control algorithm;    higher resolution images;   

DOI  :  10.1049/mnl.2019.0313

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

In the aspect of biomacromolecule imaging, high-speed atomic force microscopy (HS-AFM) has many wonderful characteristics, such as rapid imaging and real-time visualisation of the structure and dynamic behaviour of biological macromolecules, which is unparalleled by other technologies. In this review, state-of-the-art achievements on HS-AFM in molecular biology are summarised. Firstly, biomacromolecule characteristics determination and nanostructure recombination using HS-AFM are introduced, which confirm that HS-AFM is such a mature technology that realises high-speed imaging. Then, some improvements including the cantilever of HS-AFM, mechanical scanner, position sensor, and closed-loop control algorithm are reviewed to obtain higher resolution images. Finally, future development directions that could enhance the performance of HS-AFM are discussed.

【 授权许可】

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