Ethanolic Extract from Pteris wallichiana Alleviates DSS-Induced Intestinal Inflammation and Intestinal Barrier Dysfunction by Inhibiting the TLR4/NF-κB Pathway and Regulating Tight Junction Proteins

Molecules

Junhong Tao¹  Lei Tian¹  Yaping Liu¹  Guiguang Cheng¹  Tianrui Zhao¹  Yudan Wang¹  Yifen Wang²  Zhihua Huang³ 

[1] Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China;Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650000, China;Yunnan Tobacco Company, Yuxi Branch, Yuxi 653100, China;

关键词: Pteris wallichiana;    chemical constituents;    ulcerative colitis;    anti-inflammatory activity;    tight junction proteins;   

DOI  :  10.3390/molecules27103093

来源: DOAJ

【 摘 要 】

The aim of the research was to determine the protective effect and mechanism of Pteris wallichiana J. Agardh extract (PWE) on DSS-induced ulcerative colitis (UC) in mice. In this research, PWE is rich in flavonoids and diterpenoids by UPLC-MS/MS analysis. In LPS-induced RAW264.7 cells, PWE reduced the productions of inflammatory factors (i.e., NO, TNF-α, IL-6, and IL-1β). In DSS-induced UC in mice, PWE improved disease activity index (DAI) score, attenuated oxidative stress by decreasing MPO and MDA activities and activating GSH and SOD levels, and inhibited TNF-α, IL-6, and IL-1β expressions in the colonic tissues. PWE also improved the intestinal barrier by upregulating the expressions of tight junction proteins, including occludin and ZO-1. Moreover, PWE extract alleviated intestinal inflammation by suppressing the TLR4/MyD88/NF-κB signaling pathway. Conclusion: PWE can alleviate DSS-induced UC in mice by increasing the expressions of intestinal tight junction proteins and inhibiting the TLR4/NF-κB inflammatory pathway.

【 授权许可】

Unknown