Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda

African Journal of Laboratory Medicine

Sam L. Nsobya¹  Barbara S. Mensch²  Sam Kalibala³  Paul C. Hewett³ 

[1] Department of Pathology, School of Biomedical Science, Makerere University, Kampala;Population Council, New York, New York;Population Council, Washington, District of Columbia;

关键词: HSV-2;    Herpes Simplex Virus Type 2;    dried blood spots;    DBS;    Kalon;   

DOI  :  10.4102/ajlm.v5i1.429

来源: DOAJ

【 摘 要 】

This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.

【 授权许可】

Unknown