Transient Introgression of Wolbachia into Aedes aegypti Populations Does Not Elicit an Antibody Response to Wolbachia Surface Protein in Community Members

Pathogens

Le Trung Nghia¹  Thanh Dong Nguyen¹  Quang Mai Vien¹  Robson Kriiger Loterio²  Elvina Lee²  Heather A. Flores²  Johanna E. Fraser²  Nhu Duong Tran³  Sinh Nam Vu³  Tran Hien Nguyen³  Thu Yen Nguyen³  Duc Anh Dang³  Cameron P. Simmons⁴  Iñaki Iturbe-Ormaetxe⁴  Scott L. O’Neill⁴ 

[1] Institute Pasteur, Nha Trang 650000, Vietnam;Institute of Vector-Borne Disease, Monash University, Clayton, VIC 3800, Australia;National Institute of Hygiene and Epidemiology, Hanoi 100000, Vietnam;World Mosquito Program, Institute of Vector-Borne Disease, Monash University, Clayton, VIC 3800, Australia;

关键词: Wolbachia;    Aedes aegypti;    vector biology;    arbovirus;    dengue virus;   

DOI  :  10.3390/pathogens11050535

来源: DOAJ

【 摘 要 】

Wolbachia is an endosymbiotic bacterium that can restrict the transmission of human pathogenic viruses by Aedes aegypti mosquitoes. Recent field trials have shown that dengue incidence is significantly reduced when Wolbachia is introgressed into the local Ae. aegypti population. Female Ae. aegypti are anautogenous and feed on human blood to produce viable eggs. Herein, we tested whether people who reside on Tri Nguyen Island (TNI), Vietnam developed antibodies to Wolbachia Surface Protein (WSP) following release of Wolbachia-infected Ae. aegypti, as a measure of exposure to Wolbachia. Paired blood samples were collected from 105 participants before and after mosquito releases and anti-WSP titres were measured by ELISA. We determined no change in anti-WSP titres after ~30 weeks of high levels of Wolbachia-Ae. aegypti on TNI. These data suggest that humans are not exposed to the major Wolbachia surface antigen, WSP, following introgression of Wolbachia-infected Ae. aegypti mosquitoes.

【 授权许可】

Unknown