Sinomenine restrains breast cancer cells proliferation, migration and invasion via modulation of miR-29/PDCD-4 axis

Artificial Cells, Nanomedicine, and Biotechnology

Guanglei Gao¹  Wenyan Ma²  Xiaolin Liang³ 

[1] Department of Galactophore, Linyi Central Hospital, Linyi, China;Department of Pharmacy, Jining No.1 People's Hospital, Jining, China;Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China;

关键词: Sinomenine;    microRNA-29;    breast cancer;    PDCD-4;    JNK;    MEK/ERK;   

DOI  :  10.1080/21691401.2019.1666861

来源: DOAJ

【 摘 要 】

Sinomenine (Sino) is diffusely applied in heal rheumatoid arthritis and neuralgia. Howbeit, the activities of Sino in breast cancer cells remain confused. The research attempted to probe the anti-tumor function of Sino in breast cancer cells and divulge the feasible molecular mechanism. Sion at the 1–16 μM concentrations was exploited for the exposure of MDA-MB-231 or MCF7 cells, and cell growth, migration, invasion, cell cycle-relevant and apoptosis-correlative factors were estimated. Micro RNA (miR)-29 expression was evaluated via enforcing qRT-PCR, and the actions of miR-29 in MDA-MB-231 cells growth, migration and invasion were appraised after the overexpressed or suppressed vectors transfection. The functions of PDCD-4 in JNK and MEK/ERK pathways were estimated by employing western blot. We found that, Sino exposure impeded cell proliferation, provoked cell apoptosis and barricaded cell migration and invasion in MDA-MB-231 and MCF7 cells. Enhancement of miR-29 was observed in Sino-managed cells, and miR-29 overexpression further potentiated the activities of Sino in MDA-MB-231 cells. Additionally, Sino remarkably enhanced PCDC-4 expression via adjusting miR-29 in MDA-MB-231 cells. Beyond that, overexpressed PCDC-4 obstructed JNK and MEK/ERK pathways in MDA-MB-231 cells. Taken together, the explorations unveiled that Sino restrained MDA-MB-231 cells proliferation, migration, invasion, and provoked apoptosis through modulation of miR-29/PDCD-4 axis.HighlightSino inhibits MDA-MB-231 and MCF7 cells proliferation and provokes apoptosis;Sino restrains MDA-MB-231 and MCF7 cells migration and invasion;Sino ascends miR-29 expression in MDA-MB-231 and MCF7 cells;Sino adjusts cell growth, migration and invasion via modulating miR-29;Sino up-regulates PDCD-4 expression through mediating miR-29;PDCD-4 obstructs JNK and MEK/ERK pathways in MDA-MB-231 cells.

【 授权许可】

Unknown