Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains

International Journal of Molecular Sciences

Hee-Seok Kweon¹  Irina S. Sesorova²  Galina V. Beznoussenko³  Alexander A. Mironov³ 

[1] Center for Research Equipment, Korea Basic Science Institute, 169-148, Gwahak-ro, Yuseong-gu, Daejeon 34133, Korea;Department of Anatomy, Ivanovo State Medical Academy, 153462 Ivanovo, Russia;The FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy;

关键词: intracellular traffic;    Golgi complex;    membrane fusion;    Golgi dynamics;    SNAREs;    COPI;   

DOI  :  10.3390/ijms23073590

来源: DOAJ

【 摘 要 】

The Golgi complex is the central station of the secretory pathway. Knowledge about the mechanisms of intra-Golgi transport is inconsistent. Here, we compared the explanatory power of the cisterna maturation-progression model and the kiss-and-run model. During intra-Golgi transport, conventional cargoes undergo concentration and form cisternal distensions or distinct membrane domains that contain only one membrane cargo. These domains and distension are separated from the rest of the Golgi cisternae by rows of pores. After the arrival of any membrane cargo or a large cargo aggregate at the Golgi complex, the cis-Golgi SNAREs become enriched within the membrane of cargo-containing domains and then replaced by the trans-Golgi SNAREs. During the passage of these domains, the number of cisternal pores decreases. Restoration of the cisternal pores is COPI-dependent. Our observations are more in line with the kiss-and-run model.

【 授权许可】

Unknown