Effects of brefeldin A and nordihydroguaiaretic acid on endomembrane dynamics and lipid synthesis in plant cells

FEBS Letters

Perret, Anne-Marie¹  Képès, François²  Satiat-Jeunemaitre, Béatrice²  Mérigout, Patricia²  Moreau, Patrick¹ 

[1] Laboratoire de Biogenèse Membranaire, CNRS UMR 5544, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, case 92, 33076 Bordeaux Cedex, France;Laboratoire de Dynamique de la Compartimentation Cellulaire, Institut des Sciences du Végétal, CNRS UPR 2355, 91198 Gif-sur-Yvette Cedex, France

关键词: Endoplasmic reticulum;    Golgi dynamics;    Brefeldin A;    Nordihydroguaiaretic acid;    Sterol;   

DOI  :  10.1016/S0014-5793(02)02651-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effects of brefeldin A (BFA) and nordihydroguaiaretic acid (NDGA) on endomembrane structures and lipid synthesis were compared in maize root cells and tobacco Bright Yellow-2 cells. Immunofluorescence and electron microscopy studies showed that NDGA altered the structure and distribution of the endoplasmic reticulum (ER) within 1 h but not of the Golgi apparatus whereas, as shown previously, BFA altered that organization of the Golgi apparatus and, only subsequently, of the ER. Biochemical studies revealed that both drugs and especially BFA led to a strong inhibition of the phytosterol biosynthetic pathway: BFA led to accumulation of sterol precursors. The importance of phytosterols in membrane architecture and membrane trafficking is discussed.

【 授权许可】

Unknown