Reproductive Toxicity of Furfural Acetone in Meloidogyne incognita and Caenorhabditis elegans

Cells

Minmin Cai¹  Longyu Zheng¹  Wanli Cheng¹  Feng Huang¹  Hua Xue¹  Dian Huang¹  Jibin Zhang¹  Ziniu Yu¹  Xue Yang¹ 

[1] State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;

关键词: furfural acetone;    Meloidogyne incognita;    Caenorhabditis elegans;    reproductive toxicity;    germ-cell apoptosis;   

DOI  :  10.3390/cells11030401

来源: DOAJ

【 摘 要 】

Furfural acetone (FAc) is a promising alternative to currently available nematicides, and it exhibits equivalent control efficiency on root-knot nematodes with avermectin in fields. However, its effect on the reproduction of root-knot nematode is poorly understood. In this study, the natural metabolite FAc was found to exhibit reproductive toxicity on Meloidogyne incognita and Caenorhabditis elegans. The number of germ cells of C. elegans was observed to decrease after exposure to FAc, with a reduction of 59.9% at a dose of 200 mg/L. FAc in various concentrations induced the germ-cell apoptosis of C. elegans, with an increase over six-fold in the number of apoptotic germ cells at 200 mg/L. These findings suggested that FAc decreased the brood size of nematode by inducing germ-cell apoptosis. Moreover, FAc-induced germ-cell apoptosis was suppressed by the mutation of gene hus-1, clk-2, cep-1, egl-1, ced-3, ced-4, or ced-9. The expression of genes spo-11, cep-1, and egl-1 in C. elegans was increased significantly after FAc treatment. Taken together, these results indicate that nematode exposure to FAc might inflict DNA damage through protein SPO-11, activate CEP-1 and EGL-1, and induce the core apoptosis pathway to cause germ-cell apoptosis, resulting in decreased brood size of C. elegans.

【 授权许可】

Unknown