MiMIF-2 Effector of Meloidogyne incognita Exhibited Enzyme Activities and Potential Roles in Plant Salicylic Acid Synthesis

International Journal of Molecular Sciences

卷:21

Heng Jian¹  Qian Liu¹  Jianlong Zhao²  Zhenchuan Mao²  Bingyan Xie²  Qinghua Sun² 

[1] Department of Plant Pathology and Key Laboratory of Plant Pathology of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China;

[2] Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China;

关键词: Meloidogyne incognita;    effector;    MiMIF-2;    enzyme activity;    transcriptome;    salicylic acid;   

DOI  :  10.3390/ijms21103507

来源: DOAJ

【 摘 要 】

Plant-parasitic nematodes secrete a series of effectors to promote parasitism by modulating host immunity, but the detailed molecular mechanism is ambiguous. Animal parasites secrete macrophage migration inhibitory factor (MIF)-like proteins for evasion of host immune systems, in which their biochemical activities play essential roles. Previous research demonstrated that MiMIF-2 effector was secreted by Meloidogyne incognita and modulated host immunity by interacting with annexins. In this study, we show that MiMIF-2 had tautomerase activity and protected nematodes against H₂O₂ damage. MiMIF-2 expression not only decreased the amount of H₂O₂ generation during nematode infection in Arabidopsis, but also suppressed Bax-induced cell death by inhibiting reactive oxygen species burst in Nicotiana benthamiana. Further, RNA-seq transcriptome analysis and RT-qPCR showed that the expression of some heat-shock proteins was down regulated in MiMIF-2 transgenic Arabidopsis. After treatment with flg22, RNA-seq transcriptome analysis indicated that the differentially expressed genes in MiMIF-2 expressing Arabidopsis were pointed to plant hormone signal transduction, compound metabolism and plant defense. RT-qPCR and metabolomic results confirmed that salicylic acid (SA) related marker genes and SA content were significantly decreased. Our results provide a comprehensive understanding of how MiMIF-2 modulates plant immunity and broaden knowledge of the intricate relationship between M. incognita and host plants.

【 授权许可】

Unknown