Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation

International Journal of Molecular Sciences

Franca M. Nigro¹  Pasquale De Vita¹  Nicola Pecchioni¹  Romina Beleggia¹  Roberto Papa¹  Fabio Fiorani²  Ulrich Schurr²  Jacques L. David³  Yan Holtz³  Tania Gioia⁴  Zoran Nikoloski⁵  Nooshin Omranian⁵ 

[1] Council for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, Italy;Institute of Biosciences and Geosciences (IBG-2): Plant Sciences, Forschungszentrum Julich GmbH, 52428 Julich, Germany;Montpellier SupAgro, UMR Amelioration Genetique et Adaptation des Plantes, 34060 Montpellier, France;Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Università degli Studi della Basilicata, 85100 Potenza, Italy;Systems Biology and Mathematical Modeling Group, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany;

关键词: plant nutrition;    Triticum turgidum;    transcriptomics;    metabolomics;    stress;    GABA;   

DOI  :  10.3390/ijms22094790

来源: DOAJ

【 摘 要 】

Mounting evidence indicates the key role of nitrogen (N) on diverse processes in plant, including development and defense. Using a combined transcriptomics and metabolomics approach, we studied the response of seedlings to N starvation of two different tetraploid wheat genotypes from the two main domesticated subspecies: emmer and durum wheat. We found that durum wheat exhibits broader and stronger response in comparison to emmer as seen from the expression pattern of both genes and metabolites and gene enrichment analysis. They showed major differences in the responses to N starvation for transcription factor families, emmer showed differential reduction in the levels of primary metabolites while durum wheat exhibited increased levels of most of them to N starvation. The correlation-based networks, including the differentially expressed genes and metabolites, revealed tighter regulation of metabolism in durum wheat in comparison to emmer. We also found that glutamate and -aminobutyric acid (GABA) had highest values of centrality in the metabolic correlation network, suggesting their critical role in the genotype-specific response to N starvation of emmer and durum wheat, respectively. Moreover, this finding indicates that there might be contrasting strategies associated to GABA and glutamate signaling modulating shoot vs. root growth in the two different wheat subspecies.

【 授权许可】

Unknown