Overexpression of zinc-α2-glycoprotein suppressed seizures and seizure-related neuroflammation in pentylenetetrazol-kindled rats

Journal of Neuroinflammation

Xinyuan Yu¹  Xueying Ding¹  Ying Liu¹  Xin Wei¹  Xi Liu¹  Tao Xu¹  Maojia Yin¹  Xinjie Tan¹  Lijuan Mo¹  Teng Wang¹  Lifen Chen¹  Yuetao Wen² 

[1] Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University;Department of Neurosurgery, The University-Town Hospital of Chongqing Medical University;

关键词: Zinc-α2-glycoprotein;    Epilepsy;    Neuroinflammation;    TNFα;    IL-6;    TGFβ;   

DOI  :  10.1186/s12974-018-1132-6

来源: DOAJ

【 摘 要 】

Abstract Background Zinc-α2-glycoprotein (ZAG) is a 42-kDa protein reported as an anti-inflammatory adipocytokine. Evidences from clinical and experimental studies revealed that brain inflammation plays important roles in epileptogenesis and seizure. Interestingly, closely relationship between ZAG and many important inflammatory mediators has been proven. Our previous study identified ZAG in neurons and found that ZAG is decreased in epilepsy and interacts with TGFβ and ERK. This study aimed to investigate the role of ZAG in seizure and explore its effect on seizure-related neuroinflammation. Methods We overexpressed AZGP1 in the hippocampus of rats via adeno-associated virus vector injection and observed their seizure behavior and EEG after pentylenetetrazol (PTZ) kindling. The level of typical inflammation mediators including TNFα, IL-6, TGFβ, ERK, and ERK phosphorylation were determined. Results The overexpression of AZGP1 reduced the seizure severity, prolonged the latency of kindling, and alleviated epileptiform discharges in EEG changes induced by PTZ. Overexpression of AZGP1 also suppressed the expression of TNFα, IL-6, TGFβ, and ERK phosphorylaton in PTZ-kindled rats. Conclusions ZAG may inhibit TGFβ-mediated ERK phosphorylation and inhibit neuroinflammation mediated by TNFα and IL-6, suggesting ZAG may suppress seizure via inhibiting neuroinflammation. ZAG may be a potential and novel therapeutic target for epilepsy.

【 授权许可】

Unknown