Erianin against Staphylococcus aureus Infection via Inhibiting Sortase A

Toxins

Xiaoxia Liang¹  Xu Song¹  Ping Ouyang¹  Lizi Yin¹  Zhong-Wei Yuan¹  Lixia Li¹  Zhong-Qiong Yin¹  Changliang He¹  Gang Shu¹  Zhi-Xiang Yuan¹  Xuewen He¹  Hualin Fu¹  Juchun Lin¹  Cheng Lv¹ 

[1] College of Veterinary Medicine, Sichuan Agriculture University, Chengdu 610000, China;

关键词: sortase A;    Staphylococcus aureus;    erianin;    inhibitor;    molecular mechanism;   

DOI  :  10.3390/toxins10100385

来源: DOAJ

【 摘 要 】

With continuous emergence and widespread of multidrug-resistant Staphylococcus aureus infections, common antibiotics have become ineffective in treating these infections in the clinical setting. Anti-virulence strategies could be novel, effective therapeutic strategies against drug-resistant bacterial infections. Sortase A (srtA), a transpeptidase in gram-positive bacteria, can anchor surface proteins that play a vital role in pathogenesis of these bacteria. SrtA is known as a potential antivirulent drug target to treat bacterial infections. In this study, we found that erianin, a natural bibenzyl compound, could inhibit the activity of srtA in vitro (half maximal inhibitory concentration—IC50 = 20.91 ± 2.31 μg/mL, 65.7 ± 7.2 μM) at subminimum inhibitory concentrations (minimum inhibitory concentrations—MIC = 512 μg/mL against S. aureus). The molecular mechanism underlying the inhibition of srtA by erianin was identified using molecular dynamics simulation: erianin binds to srtA residues Ile182, Val193, Trp194, Arg197, and Ile199, forming a stable bond via hydrophobic interactions. In addition, the activities of S. aureus binding to fibronectin and biofilm formation were inhibited by erianin, when co-culture with S. aureus. In vivo, erianin could improve the survival in mice that infected with S. aureus by tail vein injection. Experimental results showed that erianin is a potential novel therapeutic compound against S. aureus infections via affecting srtA.

【 授权许可】

Unknown