Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical

Biomolecules

Kai Wen¹  Ling Yang¹  Dongshuai Shen¹  Zhanhui Wang¹  Xuezhi Yu¹  Jianzhong Shen¹  Yuan Li¹  Xiya Zhang² 

[1] Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, China;College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China;

关键词: fluoroacetamide;    hapten design and synthesis;    antibodies;    toxins;    immunoassay;   

DOI  :  10.3390/biom10070986

来源: DOAJ

【 摘 要 】

Fluoroacetamide (FAM) is a small (77 Da) and highly toxic chemical, formerly used as a rodenticide and potentially as a poison by terrorists. Poisoning with FAM has occurred in humans, but few reliably rapid detection methods and antidotes have been reported. Therefore, producing a specific antibody to FAM is not only critical for the development of a fast diagnostic but also a potential treatment. However, achieving this goal is a great challenge, mainly due to the very low molecular weight of FAM. Here, we design two groups of FAM haptens for the first time, maximally exposing the fluorine or amino groups, with the aid of linear aliphatic or phenyl-contained spacer arms. Interestingly, whereas the hapten with fluorine at the far end of the hapten did not induce an antibody response to FAM, the hapten with an amino group at the far end and phenyl-contained spacer arm triggered a significantly specific antibody response. Finally, a monoclonal antibody (mAb) named 5D11 was successfully obtained with an IC₅₀ value of 97 μg mL⁻¹ and negligible cross-reactivities to the other nine functional and structural analogs.

【 授权许可】

Unknown