Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes

Sibirskij Onkologičeskij Žurnal

O. P. Kutenkov¹  V. V. Rostov¹  I. N. Lebedev²  S. A. Vasilyev²  A. A. Belenko³  M. A. Bolshakov⁴ 

[1] Institute of High-Current Electronics SB RAS, Tomsk;Institute of Medical Genetics SB RAMS, Tomsk;Tomsk State University;Tomsk1, Tomsk State University, Institute of High-Current Electronics SB RAS, Tomsk;

关键词: pulsed x-rays;    low doses;    hyperradiosensitivity;    γh2ax and 53bp1 foci;    dna double-strand breaks;   

DOI  :  

来源: DOAJ

【 摘 要 】

Effects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. Thus, the aim of this study was to analyze effects of pulsed X-rays on level of radiation-induced DNA double-strand breaks and their repair kinetics in cancer and normalhuman cells in vitro. Analysis of radiation-induced γH2AX and 53BP1 repair foci in MOLT-4 cells with lymphoblastic origin was used for assessment of DNA double-strand breaks (DSB) in these cells. Number of residual radiation-induced γH2AX and 53BP1 foci at 18 hafter irradiation depended on frequency of X-ray pulses: at 8 pulses per second effect was highest in MOLT-4 cells and lowest in peripheral blood lymphocytes. It suggests that pulsed X-rays with various frequencies could be used for target influence on cancer cells being lessdeleterious for normal human cells.

【 授权许可】

Unknown