Basic Fibroblast Growth Factor 2-Induced Proteome Changes Endorse Lewy Body Pathology in Hippocampal Neurons

iScience

Stefan F. Lichtenthaler¹  Stephan A. Müller²  Sainitin Donakonda³  Thomas Koeglsperger⁴  Rohit Kumar⁴  Günter U. Höglinger⁴  Kai Bötzel⁵ 

[1] Corresponding author;Department of Neurology, Ludwig Maximilian University, 81377 Munich, Germany;Faculty of Medicine, Klinikum rechts der Isar, Technical University of Munich, 81675 Munich, Germany;German Center for Neurodegenerative Diseases (DZNE), 81337 Munich, Germany;Institute of Immunology and Experimental Oncology, Technical University of Munich, 81675 Munich, Germany;

关键词: Biological Sciences;    Molecular Neuroscience;    Proteomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Hippocampal Lewy body pathology (LBP) is associated with changes in neurotrophic factor signaling and neuronal energy metabolism. LBP progression is attributed to the aggregation of α-synuclein (α-Syn) and its cell-to-cell transmission via extracellular vehicles (EVs). We recently discovered an enhanced EV release in basic fibroblast growth factor (bFGF)-treated hippocampal neurons. Here, we examined the EV and cell lysate proteome changes in bFGF-treated hippocampal neurons. We identified n = 2,310 differentially expressed proteins (DEPs) induced by bFGF. We applied weighted protein co-expression network analysis (WPCNA) to generate protein modules from DEPs and mapped them to published LBP datasets. This approach revealed n = 532 LBP-linked DEPs comprising key α-Syn-interacting proteins, LBP-associated RNA-binding proteins (RBPs), and neuronal ion channels and receptors that can impact LBP onset and progression. In summary, our deep proteomic analysis affirms the potential influence of bFGF signaling on LBP-related proteome changes and associated molecular interactions.

【 授权许可】

Unknown