Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects

Polymers

A. Sivashanmugam¹  R. Jayakumar¹  MiYeon Noh²  SeongKeun Kwon²  SeunghunS. Lee²  Janet Kwon²  SeungHyun L. Kim²  Song Kwon³  NathanielS. Hwang³ 

[1] Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi 682041, India;Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Korea;School of Chemical and Biological Engineering, the Institute of Chemical Processes, Seoul National University, Seoul 08826, Korea;

关键词: methacrylated gelatin;    bioglass;    hydrogel;    cryogel;    bone tissue engineering;   

DOI  :  10.3390/polym10080914

来源: DOAJ

【 摘 要 】

Cryogels have recently gained interest in the field of tissue engineering as they inherently possess an interconnected macroporous structure. Considered to be suitable for scaffold cryogel fabrication, methacrylated gelatin (GelMA) is a modified form of gelatin valued for its ability to retain cell adhesion site. Bioglass nanoparticles have also attracted attention in the field due to their osteoinductive and osteoconductive behavior. Here, we prepare methacrylated gelatin cryogel with varying concentration of bioglass nanoparticles to study its potential for bone regeneration. We demonstrate that an increase in bioglass concentration in cryogel leads to improved mechanical property and augmented osteogenic differentiation of mesenchymal cells during in vitro testing. Furthermore, in vivo testing in mice cranial defect model shows that highest concentration of bioglass nanoparticles (2.5 w/w %) incorporated in GelMA cryogel induces the most bone formation compared to the other tested groups, as studied by micro-CT and histology. The in vitro and in vivo results highlight the potential of bioglass nanoparticles incorporated in GelMA cryogel for bone regeneration.

【 授权许可】

Unknown