Metal-chelated cryogels for amyloglucosidase adsorption: application for continuous starch hydrolysis

Bulletin of Materials Science

SINEM EVLI^1¹  HANDE ORHAN^1²  PELIN SÖZEN AKTAS^2³ 

[1] Faculty of Science and Arts, Chemistry Division, Adnan Menderes University, Aydın, Turkey^1;Faculty of Science and Arts, Chemistry Division, Celal Bayar University, Manisa, Turkey^2;Nanotechnology Application and Research Center, Adnan Menderes University, Aydın, Turkey^3

关键词: Amyloglucosidase;    cryogel;    starch hydrolysis.;   

DOI  :  

学科分类：材料工程

来源: Indian Academy of Sciences

【 摘 要 】

In the present work, a newmetal-chelating platformwas designed by using IDA as a chelating agent and Cu(II) as an affinity component for amyloglucosidase adsorption. Poly(AAm-GMA) cryogels were used as structural elements, whileGMA monomer served reactive epoxy groups for IDA immobilization. Synthesized cryogels were characterized by FTIR, SEM and EDX studies. Pore diameter of the whole polymeric structure was 3–10 $\mu$m. Effects of medium pH, temperature,ionic strength along with amyloglucosidase concentrationwere also investigated for more effective amyloglucosidase adsorption and maximum adsorbed amount of amyloglucosidase was 2.93 mg g$^{−1}$ cryogel by the optimum conditions. Reusability profile of the poly(AAm-GMA)-IDA-Cu$^{2+}$ cryogels was also studied and it was found that the synthesized cryogels could be used repeatedly for many times without any significant decrease on their adsorption capacity. Also continuous hydrolysis of starch by using immobilized form of amyloglucosidase in a column system was studied.

【 授权许可】

CC BY