Engineered Polyploid Yeast Strains Enable Efficient Xylose Utilization and Ethanol Production in Corn Hydrolysates

Frontiers in Bioengineering and Biotechnology

Sajid Ali¹  Meilin Kong¹  Yixuan Zhu¹  Zefang Jia¹  Lulu Liu¹  Limin Cao¹  Wei Xiao²  Yingqian Kang³  Wenjie Yuan⁴  Mingjie Jin⁵  Zhaoxian Xu⁵  Mingtao Huang⁶ 

[1] Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing, China;Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada;Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China;School of Bioengineering, Dalian University of Technology, Dalian, China;School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, China;School of Food Science and Engineering, South China University of Technology, Guangzhou, China;

关键词: Saccharomyces cerevisiae;    mutation;    triploid;    hydrolysate;    ethanol;   

DOI  :  10.3389/fbioe.2021.655272

来源: DOAJ

【 摘 要 】

The reported haploid Saccharomyces cerevisiae strain F106 can utilize xylose for ethanol production. After a series of XR and/or XDH mutations were introduced into F106, the XR-K270R mutant was found to outperform others. The corresponding haploid, diploid, and triploid strains were then constructed and their fermentation performance was compared. Strains F106-KR and the diploid produced an ethanol yield of 0.45 and 0.48 g/g total sugars, respectively, in simulated corn hydrolysates within 36 h. Using non-detoxicated corncob hydrolysate as the substrate, the ethanol yield with the triploid was approximately sevenfold than that of the diploid at 40°C. After a comprehensive evaluation of growth on corn stover hydrolysates pretreated with diluted acid or alkali and different substrate concentrations, ethanol yields of the triploid strain were consistently higher than those of the diploid using acid-pretreatment. These results demonstrate that the yeast chromosomal copy number is positively correlated with increased ethanol production under our experimental conditions.

【 授权许可】

Unknown