Mycotoxin binder improves growth rate in piglets associated with reduction of toll-like receptor-4 and increase of tight junction protein gene expression in gut mucosa

Journal of Animal Science and Biotechnology

Honglin Yan¹  Noémie Van Noten¹  Joris Michiels¹  Wei Wang¹  Maryam Majdeddin¹  Linghong Jin¹  Jeroen Degroote¹  Mario Van Poucke²  Luc Peelman²  Stefaan De Smet³  Ronny Mombaerts⁴  Anne Goderis⁴  Kurt Van De Mierop⁴ 

[1] Department of Applied Biosciences, Ghent University;Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University;Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University;Nutrex;

关键词: Binder;    Deoxynivalenol;    Gut barrier;    Gut health;    Mycotoxin;    Pigs;   

DOI  :  10.1186/s40104-017-0210-4

来源: DOAJ

【 摘 要 】

Abstract Background Deoxynivalenol (DON) is a mycotoxin produced by Fusarium species in the field, commonly found in cereal grains, which negatively affects performances and health of animals. Mycotoxin binders are supposed to reduce the toxicity of mycotoxins. Method The effect of a mycotoxin binder (containing acid-activated bentonite, clinoptilolite, yeast cell walls and organic acids) on growth performance and gut health was studied. Hundred and twenty weaning piglets were allocated to 4 treatments, with 5 pens of 6 piglets each, arranged in a 2 × 2 factorial design: control diet; control diet with 1 kg/t binder; control diet with DON; and control diet with DON and 1 kg/t binder. From d0–14, the diet of DON-challenged groups was artificially contaminated with a mixture of DON (2.6 mg/kg), 3-acetyl-deoxynivalenol (0.1 mg/kg) and 15-acetyl-deoxynivalenol (0.3 mg/kg), after which the total contamination level was reduced to 1 mg/kg, until d37. On d14, one pig from each pen was euthanized and distal small intestinal mucosa samples were collected for the assessment of intestinal permeability, and gene expression of tight junction proteins, toll-like receptor 4, inflammatory cytokines and intestinal alkaline phosphatase. Results After 37 d, there were no differences in growth performance between control and DON-challenged groups (P > 0.05). Nevertheless, groups that received diets with binder had a significantly higher average daily gain (ADG) and average daily feed intake (ADFI) for the first 14 d as well as for the whole period, compared to groups without binder (P ≤ 0.05). Groups with binder in the diet also exhibited lower expression of toll-like receptor 4 in distal small intestinal mucosa at d14, compared to groups without binder (P ≤ 0.05). Interestingly, comparing the two DON treatments, piglets fed DON and binder had significantly higher ADFI and ADG compared to those with only DON for the first 14-d (P ≤ 0.05). Addition of binder to DON contaminated diets, also down-regulated the gene expression of toll-like receptor 4 (P ≤ 0.05) and increased mRNA level zona occludens 1 (P ≤ 0.10) as compared to DON. Conclusions The present data provide evidence that the binder improves growth rate in piglets associated with reduction of toll-like receptor-4 and increase of tight junction protein gene expression. However, the current study does not allow to assess whether the effects of the binder are mediated by alterations in the toxicokinetics of the mycotoxin.

【 授权许可】

Unknown