Morin Protects Human Respiratory Cells from PM2.5 Induced Genotoxicity by Mitigating ROS and Reverting Altered miRNA Expression

International Journal of Environmental Research and Public Health

Indhumathi Veerappan¹  Rajaguru Palanisamy¹  SenthilKumar Sankareswaran¹ 

[1] Department of Biotechnology, Anna University, BIT Campus, Tiruchirappalli 620 024, India;

关键词: Particulate matter;    cytotoxicity;    genotoxicity;    comet assay;    oxidative stress;    miRNA;    morin;   

DOI  :  10.3390/ijerph16132389

来源: DOAJ

【 摘 要 】

Chronic fine particulate matter (PM_2.5) exposure causes oxidative stress and leads to many diseases in human like respiratory and cardiovascular disorders, and lung cancer. It is known that toxic responses elicited by PM_2.5 particles depend on its physical and chemical characteristics that are greatly influenced by the source. Dietary polyphenolic compounds that possess antioxidant and free radical scavenging properties could be used for therapeutic or preventive approaches against air pollution related health hazards. This study evaluates characteristics and toxicity of PM_2.5 collected from rural, urban, industrial, and traffic regions in and around Coimbatore City, Tamilnadu, India. Traffic PM_2.5 particles contained higher amounts of metals and polycyclic aromatic hydrocarbons (PAHs). It also possessed higher levels of oxidative potential, induced more intracellular reactive oxygen species (ROS), and caused more levels of cell death and DNA damage in human respiratory cells. Its exposure up regulated DNA damage response related miR222, miR210, miR101, miR34a, and miR93 and MycN and suppressed Rad52. Pre-treatment with morin significantly decreased the PM_2.5 induced toxicity and conferred protection against PM_2.5 induced altered miRNA expression. Results of this study showed that cytoprotective effect of morin is due to its antioxidative and free radical scavenging activity.

【 授权许可】

Unknown