Profile of micronucleus frequencies and DNA damage in different species of fish in a eutrophic tropical lake

Genetics and Molecular Biology

Cesar K. Grisolia¹  Carla L. G. Rivero¹  Fernando L. R. M. Starling¹  Izabel C. R. Da Silva¹  Antonio C. Barbosa¹  Jose G. Dorea¹ 

关键词: genotoxicity;    fish biomonitoring;    eutrophic lake;    micronucleus;    comet assay;    cytotoxicity;   

DOI  :  10.1590/S1415-47572009005000009

来源: SciELO

【 摘 要 】

Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis ,Cichla temensis ,Hoplias malabaricus ,Astyanax bimaculatus lacustres ,Oreochromis niloticus ,Cyprinus carpioandSteindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found inCichla temensisandHoplias malabaricus , which were statistically significant when compared to the other species. However,Steindachnerina insculpita(a detritivorous and lake-floor feeder species) showed the highest index of DNA damage in the comet assay, followed byC. temensis(piscivorous). Nuclear abnormalities, such as binucleated, blebbed, lobed and notched cells, were used as evidence of cytotoxicity.Oreochromis niloticusfollowed byHoplias malaricus , ominivorous/detritivotous and piscivorous species, respectively, presented the highest frequency of nuclear abnormalities, especially notched cells, while the herbivorousAstyanax bimaculatus lacustresshowed the lowest frequency compared to the other species studied. Thus, for biomonitoring aquatic genotoxins under field conditions, the food web should also be considered.

【 授权许可】

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