Deep proteomic dataset of human liver samples obtained by two-dimensional sample fractionation coupled with tandem mass spectrometry

Data in Brief

Ekaterina V. Ilgisonis¹  Nikita E. Vavilov²  Alexander I. Archakov³  Olga V. Tikhonova³  Andrey V. Lisitsa³  Victor G. Zgoda³  Elena A. Ponomarenko³  Tatiana E. Farafonova³ 

[1] Institute of Biomedical Chemistry, Pogodinskaya street 10, bld. 7, Moscow, Russia;Corresponding author.;Institute of Biomedical Chemistry, Pogodinskaya street 10, bld. 7, Moscow, Russia;

关键词: Mass-spectrometry;    Proteomics;    Shotgun;    SRM SIS;    Fractionation;   

DOI  :  

来源: DOAJ

【 摘 要 】

The data was acquired from 3 normal human liver tissues by LC-MS methods. The tissue liver samples from male subjects post mortem were obtained from ILSBio LLC (https://bioivt.com/). Liver tissue was frozen in liquid nitrogen, transported and shipped on dry ice. The proteins were extracted and purified followed up by trypsin hydrolysis. The peptide mixture was aliquoted and analyzed by different LC-MS approaches: one-dimensional shotgun LC-MS, two-dimensional LC-MS, two-dimensional SRM SIS (Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards). The Shotgun assay resulted in a qualitative in-depth human liver proteome, and a semi-quantitative iBAQ (intensity-based absolute quantification) value was calculated to show the relative protein content of the sample. Absolute quantitative concentrations of proteins encoded by human chromosome 18 using SRM SIS were obtained.

【 授权许可】

Unknown