Anti-proliferative effect of main dietary phytosterols and β-cryptoxanthin alone or combined in human colon cancer Caco-2 cells through cytosolic Ca+2 – and oxidative stress-induced apoptosis

Journal of Functional Foods

Alessandro Attanzio¹  Reyes Barberá²  Luisa Tesoriere³  Antonio Cilla⁴  Maria A. Livrea⁴ 

[1] Corresponding author. Nutrition and Food Science Area. Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 – Burjassot (Valencia), Spain. Tel.: [phone];fax: [phone].;Dipartimento Scienze e Technologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Palermo, Italy;Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, Burjassot (Valencia) 46100, Spain;

关键词: Anti-proliferation;    Apoptosis;    β-Cryptoxanthin;    Caco-2 cells;    Phytosterols;   

DOI  :  

来源: DOAJ

【 摘 要 】

β-cryptoxanthin (β-Cx) and phytosterols (Ps) have potential against different cancer types, including colon cancer. However, their combined action has not been reported so far. Human colon cancer Caco-2 cells were treated 24 h with β-Cx and/or main dietary Ps (β-sitosterol, campesterol and stigmasterol), alone or in combination, at concentrations compatible with physiological human serum levels. A decrease in cell viability due to apoptosis (rise in sub-G1 population and exposure of membrane phosphatidylserine) was accompanied with dephosphorylation of BAD, mitochondrial depolarization and caspase 3-dependent PARP cleavage, with intracellular Ca2+ influx and increase of RONS levels as initial triggers. Ps and β-Cx, alone or in combination showed anti-proliferative activity against human colon adenocarcinoma Caco-2 cells through the mitochondrial pathway of apoptosis. No additive or synergistic effects were observed. The importance of bioactivity-guided assays with mixtures of dietary bioactive compounds to determine their eventual interactions in the functional food context is demonstrated.

【 授权许可】

Unknown