Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

International Journal of Molecular Sciences

Ya Luo¹  Qing Chen¹  Yong Zhang¹  Yuanxiu Lin¹  Xiaorong Wang¹  Bo Sun¹  Mengwen Luo¹  Yunting Zhang¹  Lijuan Cheng¹  Mengyao Li¹  Yan Wang¹  Haoru Tang¹  Xianjie Gu² 

[1] College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;Mianyang Academy of Agricultural Sciences, Mianyang 621000, China;

关键词: Glucose-6-phosphate dehydrogenase;    Cold stress;    Strawberry;    Antioxidant enzymes;    Oxidative damage;   

DOI  :  10.3390/ijms21197322

来源: DOAJ

【 摘 要 】

Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.

【 授权许可】

Unknown