Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi

Frontiers in Immunology

Santuza M. R. Teixeira¹  Alexandre Morrot³  Danielle Oliveira Nascimento⁴  Paulo M. Bisch⁴  Carolina M. Koeller⁴  Leonardo Freire-de-Lima⁴  George A. DosReis⁴  Célio Geraldo Freire-de-Lima⁴  Norton Heise⁴  Marcela F. Lopes⁴  Fabricio Montalvão⁴  Rita Vasconcellos⁵  Marise P. Nunes⁶  Leticia Miranda S. Lery⁷ 

[1] Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;Faculdade de Medicina de Petrópolis (FMP-FASE), Petrópolis, Brazil;Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil;Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil;Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil;

关键词: Trypanosoma cruzi;    antibody repertoires;    beta-tubulin;    lymphocyte activation;    Chagas disease;   

DOI  :  10.3389/fimmu.2018.00671

来源: DOAJ

【 摘 要 】

Few studies investigate the major protein antigens targeted by the antibody diversity of infected mice with Trypanosoma cruzi. To detect global IgG antibody specificities, sera from infected mice were immunoblotted against whole T. cruzi extracts. By proteomic analysis, we were able to identify the most immunogenic T. cruzi proteins. We identified three major antigens as pyruvate phosphate dikinase, Hsp-85, and β-tubulin. The major protein band recognized by host IgG was T. cruzi β-tubulin. The T. cruzi β-tubulin gene was cloned, expressed in E. coli, and recombinant T. cruzi β-tubulin was obtained. Infection increased IgG reactivity against recombinant T. cruzi β-tubulin. A single immunization of mice with recombinant T. cruzi β-tubulin increased specific IgG reactivity and induced protection against T. cruzi infection. These results indicate that repertoire analysis is a valid approach to identify antigens for vaccines against Chagas disease.

【 授权许可】

Unknown