Effects of naringenin on renal expression of organic cation transporter 1 and 2 proteins and metformin disposition in diabetic rats

Journal of Functional Foods

M. Faadiel Essop¹  Peter Mark Oroma Owira²  Edith Pascale Mofo Mato³ 

[1] Corresponding author.;Cardio-Metabolic Research Group (CMRG), Department of Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa;Molecular and Clinical Pharmacology Research Laboratory, Department of Pharmacology, Discipline of Pharmaceutical Sciences, School of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa;

关键词: Renal tubular secretion;    Metformin;    Naringenin;    Diabetes;    OCT1;    OCT2;   

DOI  :  

来源: DOAJ

【 摘 要 】

Modulation of metformin transporters could affect its pharmacokinetics/pharmacodynamics. The effects of naringenin on the renal tubular Organic Cation Transporter 1 (OCT1) and 2 (OCT2) protein expressions and subsequently the metformin clearance in diabetic rats are hereby investigated. Male Sprague Dawley rats were divided into 7 groups. Animals were daily treated orally with metformin (250 mg/kg) or naringenin (60 mg/kg) alone or both for 56 days. Blood glucose tests were done. Serum, urine, and kidney samples were collected. Naringenin alone or in combination with metformin significantly increased creatinine clearance and lowered plasma metformin concentrations in diabetic rats compared to controls. OCT1 and OCT2 protein expressions in renal tissues were unchanged in diabetic rats treated with either naringenin or metformin, respectively. However, co-administration of metformin and naringenin upregulated OCT2 expression in diabetic rats. Naringenin may stimulate renal uptake/excretion of metformin by increasing OCT2 protein expression which facilitates metformin clearance in diabetic rats.

【 授权许可】

Unknown