Selection of Newly Identified Growth-Promoting Archaea Haloferax Species With a Potential Action on Cobalt Resistance in Maize Plants

Frontiers in Plant Science

Hamada Abdelgawad²  Nashwa Hagagy³  Nosheen Akhtar⁴  Anis Ali Shah⁶  Mona Warrad⁷  Eman El Azab⁷  Samy Selim⁸  Awadh Alanazi⁸  Mohammed Yagoub Mohammed Elamir⁸  Mohamed Abdel-Mawgoud⁹  Soad K. Al Jaouni^1,10  Mohammad M. Al-Sanea^1,11 

[1] 0Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Antwerpen, Belgium;1Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt;Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt;Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan;Department of Biology, College of Science and Arts at Khulis, University of Jeddah, Jeddah, Saudi Arabia;Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences at Al-Quriat, Jouf University, Al-Quriat, Saudi Arabia;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia;Department of Medicinal and Aromatic Plants, Desert Research Centre, Cairo, Egypt;Hematology/Pediatric Oncology, Yousef Abdulatif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia;Pharmaceutical Chemistry Department, College of Pharmacy, Jouf University, Sakaka, Saudi Arabia;

关键词: archaea;    Haloferax sp;    cobalt contamination;    growth promoting;    extreme growth condition;    stress;   

DOI  :  10.3389/fpls.2022.872654

来源: DOAJ

【 摘 要 】

Soil contamination with cobalt (Co) negatively impacts plant growth and production. To combat Co toxicity, plant growth-promoting microorganisms for improving plant growth are effectively applied. To this end, unclassified haloarchaeal species strain NRS_31 (OL912833), belonging to Haloferax genus, was isolated, identified for the first time, and applied to mitigate the Co phytotoxic effects on maize plants. This study found that high Co levels in soil lead to Co accumulation in maize leaves. Co accumulation in the leaves inhibited maize growth and photosynthetic efficiency, inducing oxidative damage in the tissue. Interestingly, pre-inoculation with haloarchaeal species significantly reduced Co uptake and mitigated the Co toxicity. Induced photosynthesis improved sugar metabolism, allocating more carbon to defend against Co stress. Concomitantly, the biosynthetic key enzymes involved in sucrose (sucrose-P-synthase and invertases) and proline (pyrroline-5- carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR)) biosynthesis significantly increased to maintain plant osmotic potential. In addition to their osmoregulation potential, soluble sugars and proline can contribute to maintaining ROS hemostasis. Maize leaves managed their oxidative homeostasis by increasing the production of antioxidant metabolites (such as phenolics and tocopherols) and increasing the activity of ROS-scavenging enzymes (such as POX, CAT, SOD, and enzymes involved in the AsA/GSH cycle). Inside the plant tissue, to overcome heavy Co toxicity, maize plants increased the synthesis of heavy metal-binding ligands (metallothionein, phytochelatins) and the metal detoxifying enzymes (glutathione S transferase). Overall, the improved ROS homeostasis, osmoregulation, and Co detoxification systems were the basis underlying Co oxidative stress, mitigating haloarchaeal treatment's impact.

【 授权许可】

Unknown