Biodegradable Poly(Amino Ester) with Aromatic Backbone as Efficient Nonviral Gene Delivery Vectors

Molecules

Rong-Chuan Su¹  Qiang Liu¹  Zhi-Gang Zhao¹  Wen-Jing Yi¹ 

[1] College of Chemistry and Environmental Protection Engineering, Southwest Minzu University, Chengdu 610041, China;

关键词: gene delivery;    non-viral vectors;    aromatic backbone;    cationic polymer;    biodegradable;   

DOI  :  10.3390/molecules22040566

来源: DOAJ

【 摘 要 】

The development of gene delivery vectors with high efficiency and biocompatibility is one of the critical points of gene therapy. Two biodegradable poly(amino ester)s were synthesized via ring-opening polymerization between low molecular weight (LMW) PEI and diepoxide. The molecular weights of poly(amino ester)s were measured by GPC. Agarose gel retardation assays showed that these materials have good DNA-binding ability and can retard the electrophoretic mobility of plasmid DNA (pDNA) at a weight ratio of 1. The formed polyplexes have proper sizes of around 200 nm and zeta-potential values of about 30–40 mV for cellular uptake. In vitro experiments revealed that polymer P2 gave higher transfection efficiency than PEI 25KDa and Lipofectamine 2000 with less toxicity, especially in 293 cells. Results demonstrate that such a type of degradable poly(amino ester) may serve as a promising non-viral gene vector.

【 授权许可】

Unknown