Pseudogene DUXAP8 Promotes Cell Proliferation and Migration of Hepatocellular Carcinoma by Sponging MiR-490-5p to Induce BUB1 Expression

Frontiers in Genetics

Chunxi Zheng¹  Haiping Zhang²  Kaiqiu Chu²  Lisheng Ren²  Runhua Tian² 

[1] Department of Clinical Laboratory, Qingdao Women and Children’s Hospital, Qingdao, China;Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, China;

关键词: Hepatocellular Carcinoma;    long non-coding RNA;    bioinformatics;    regulatory mechanism;    target;   

DOI  :  10.3389/fgene.2020.00666

来源: DOAJ

【 摘 要 】

Hepatocellular Carcinoma (HCC) currently remains one of the most lethal malignancies worldwide. Recently, long non-coding RNAs (lncRNAs) had been demonstrated to play a crucial role in the progression of multiple human cancers, including HCC. In this study, we found that lncRNA DUXAP8 was upregulated in tumor samples and served as an oncogene in HCC. Bioinformatics analysis showed that DUXAP8 was significantly associated with the regulation of centrosome organization, homologous recombination, meiotic cell cycle process, sister chromatid segregation, nuclear chromosome segregation, and RNA export from the nucleus. The knockdown of DUXAP8 significantly suppresses cell proliferation and the cell cycle but induces cell apoptosis in HCC. Mechanically, the present study showed that DUXAP8 serves as a sponge of MiR-490-5p to promote the expression of BUB1 in HCC. Although the underlying regulatory mechanisms of DUXAP8 in HCC require further investigation, this study, for the first time, showed that DUXAP8 can serve as a new therapeutic target for HCC.

【 授权许可】

Unknown