Determination of Kanamycin by High Performance Liquid Chromatography

Molecules

Li Li¹  Yun Wang¹  Hualin Yang¹  Qinghua Wu¹  Xingping Zhang¹  Jiujun Wang¹ 

[1] College of Life Science, Yangtze University, Jingzhou 434025, China;

关键词: Kanamycin;    HPLC;    sample pre-treatment;    different detectors;    food contamination;   

DOI  :  10.3390/molecules24101902

来源: DOAJ

【 摘 要 】

Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practical tool. This paper presents a review of the application of HPLC analysis of kanamycin in different sample matrices. The different detectors coupled with HPLC, including Ultraviolet (UV)/Fluorescence, Evaporative Light Scattering Detector (ELSD)/Pulsed Electrochemical Detection (PED), and Mass Spectrometry, are discussed. Meanwhile, the strengths and weaknesses of each method are compared. The pre-treatment methods of food samples, including protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are also summarized in this paper.

【 授权许可】

Unknown