Captive Green Iguana Carries Diarrheagenic Escherichia coli Pathotypes

Frontiers in Veterinary Science

Jorge E. Vidal¹  María Angela Oliva-Llaven²  Carlos Ibarra-Martínez²  Gerardo Uriel Bautista-Trujillo²  Paula Mendoza-Nazar²  Leonel Mandujano-García²  Benigno Ruiz-Sesma²  Carlos Tejeda-Cruz²  Federico Antonio Gutiérrez-Miceli³  Javier Gutiérrez-Jiménez⁴  Jesús Eduardo Pérez-Batrez⁴  Liset Candelaria Pérez-Vázquez⁴ 

[1]Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, United States

[2]Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Chiapas, Tuxtla Gutiérrez, Mexico

[3]Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico

[4]Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, Tuxtla Gutiérrez, Mexico

[5]Rollins School of Public Health, Emory University, Atlanta, GA, United States

关键词: diarrheagenic Escherichia coli;    prevalence;    Iguana;    Chiapas;    antibiotic resistance;    zoonosis;   

DOI  :  10.3389/fvets.2020.00099

来源: DOAJ

【 摘 要 】

The green iguana appears to be a carrier for bacteria causing gastrointestinal infections in humans. The presence of diarrheagenic E. coli (DEC) pathotypes, however, has not been studied in this reptile. The aim of the current work was to investigate the prevalence of DEC in the intestines of 240 captive green iguanas, their phylogenetic groups, and the antibiotic susceptibility profile. E. coli strains were isolated from 41.7% (N = 100/240) of the intestinal content of green iguanas. DEC strains was identified in 25.9% of the screened population and were detected in the majority (62%, p = 0.009) of those reptiles carrying E. coli strains. Among DEC strains, STEC strains carrying the stx1 gene were the most prevalent pathotype isolated (38.7%), followed by EAEC and ETEC (27.4% each). Genetic markers of DEC strains belonging to the EHEC pathotype were not detected. More than a half of DEC strains were classified into the Clade I-II phylogroup (64.5%), followed by the phylogroup A (14.5%). The antibiotic susceptibility method demonstrated that a high proportion of DEC strains were resistance, or non-susceptible, to carbenicillin, amikacin, and ampicillin. We conclude that the green iguana kept in captivity is a carrier of DEC strains bearing resistance to first-line antibiotics, including penicillins. Given the increase presence of the green iguana in Latin American households, these reptiles represent a potential source of transmission to susceptible humans and therefore a potential source of gastrointestinal disease.

【 授权许可】

Unknown