Isolation of the highly pathogenic and zoonotic agent Burkholderia pseudomallei from a pet green Iguana in Prague, Czech Republic

BMC Veterinary Research

Falk Melzer³  Katja Mertens³  Hosny El-Adawy³  Ivonne Stamm¹  Jan Hnizdo²  Mandy C Elschner³ 

[1] Vet Med Labor GmbH, Division of IDEXX Laboratories, Mörikestrasse 28/3, Ludwigsburg, 71636, Germany;Animal Clinic, Bílá Hora, Cistovicka 44, Prague 6, 16300, Czech Republic;Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, Jena, 07743, Germany

关键词: Iguana;    Zoonoses;    Burkholderia pseudomallei;    Melioidosis;   

Others  :  1103381
DOI  :  10.1186/s12917-014-0283-7

 received in 2014-08-20, accepted in 2014-11-18,  发布年份 2014

【 摘 要 】

Background

Melioidosis caused by Burkholderia (B.) pseudomallei is an endemic zoonotic disease mainly reported from northern Australia and Southeast Asia. In Europe, cases of human melioidosis have been reported only from patients travelling to endemic regions. Besides humans, B. pseudomallei has a very broad host range in domestic and wild animals. There are some reports about importation of B. pseudomallei-infected animals from endemic areas into Europe. The present report describes the first case of B. pseudomallei infection of a pet iguana in Europe.

Case presentation

In a 5-year-old pet Iguana iguana living in a private household in Prague, Czech Republic, B. pseudomallei was isolated from pus of an abscess. The isolate VB976100 was identified by Vitek®2, MALDI-TOF mass spectrometry and polymerase chain reaction as B. pseudomallei. The molecular typing resulted in multi-locus sequence type 436 hitherto, which has been found only once worldwide in a B. pseudomallei strain isolated in the USA and originating from Guatemala. The identification as internal transcribed spacer type G indicates a close relatedness to strains mainly isolated in the Western Hemisphere. These findings support the hypothesis that the iguana became infected in this region or in a breeding facility through contact to other infected animals.

Conclusions

The present case highlights the risk of importation of the highly pathogenic and zoonotic B. pseudomallei into non-endemic regions through animal trade. Therefore, veterinarians treating animals from these areas and physicians examining patients owning such animals should include melioidosis in differential diagnosis whenever specific symptoms appear. Furthermore, veterinary authorities responsible for supervision of traders and pet shops should be aware of this risk of zoonotic transmission.

【 授权许可】

   
2014 Elschner et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Limmathurotsakul D, Peacock SJ: Melioidosis: a clinical overview. Br Med Bull 2011, 99:125-139.
• [2]Katangwe T, Purcell J, Bar-Zeev N, Denis B, Montgomery J, Alaerts M, Heyderman RS, Dance DA, Kennedy N, Feasey N, Moxon CA: Human melioidosis, Malawi, 2011. Emerg Infect Dis 2013, 19(6):981-984.
• [3]Aardema H, Luijnenburg EM, Salm EF, Bijlmer HA, Visser CE, Van’t Wout JW: Changing epidemiology of melioidosis? a case of acute pulmonary melioidosis with fatal outcome imported from Brazil. Epidemiol Infect 2005, 133(5):871-875.
• [4]Cuadros J, Gil H, Miguel JD, Marabe G, Gomez-Herruz TA, Lobo B, Marcos R, Anda P: Case report: melioidosis imported from West Africa to Europe. Am J Trop Med Hyg 2011, 85(2):282-284.
• [5]Cheng AC, Currie BJ: Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 2005, 18(2):383-416.
• [6][https://ispub.com/IJVM/2/2/5750] webcite Ouadah A, Zahedi D, Perumal R: Animal melioidosis surveillance in Sabah.Internet J Vet Med 2006, 2(2). .
• [7]Sprague LD, Neubauer H: Melioidosis in animals: a review on epizootiology, diagnosis and clinical presentation. J Vet Med B Infect Dis Vet Public Health 2004, 51(7):305-320.
• [8]Zehnder AM, Hawkins MG, Koski MA, Lifland B, Byrne BA, Swanson AA, Rood MP, Gee JE, Elrod MG, Beesley CA, Blaney DD, Ventura J, Hoffmaster AR, Beeler ES: Burkholderia pseudomallei isolates in 2 pet iguanas, California, USA. Emerg Infect Dis 2014, 20(2):304-306.
• [9]Galyov EE, Brett PJ, DeShazer D: Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis. Annu Rev Microbiol 2010, 64:495-517.
• [10]Ashdown LR: An improved screening technique for the isolation of Pseudomonas pseudomallei from clinical specimens. Pathology 1979, 11:293-297.
• [11]Tomaso H, Scholz HC, Al Dahouk S, Pitt TL, Treu TM, Neubauer H: Development of 5’ nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex. Diagn Mol Pathol 2004, 13(4):247-253.
• [12]Novak RT, Glass MB, Gee JE, Gal D, Mayo MJ, Currie BJ, Wilkins PP: Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei. J Clin Microbiol 2006, 44(1):85-90.
• [13]Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, Spratt BG: Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 2003, 41(5):2068-2079.
• [14]Aanensen DM, Spratt BG: The multilocus sequence typing network: mlst.net. Nucleic Acids Res 2005, 33(Web Server issue):W728-W733.
• [15]Gee JE, Allender CJ, Tuanyok A, Elrod MG, Hoffmaster AR: Burkholderia pseudomallei type G in Western Hemisphere. Emerg Infect Dis 2014, 20(4):682-684.
• [16]Liguori AP, Warrington SD, Ginther JL, Pearson T, Bowers J, Glass MB, Mayo M, Wuthiekanun V, Engelthaler D, Peacock SJ, Currie BJ, Wagner DM, Keim P, Tuanyok A: Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors. PLoS One 2011, 6(12):e29323.
• [17]Sutmoller P, Kraneveld FC, Van Der Schaaf A: Melioidosis (Pseudomalleus) in sheep, goats, and pigs on Aruba (Netherland Antilles). J Am Vet Med Assoc 1957, 130(9):415-417.
• [18]Dance DA: Melioidosis: the tip of the iceberg? Clin Microbiol Rev 1991, 4(1):52-60.
• [19]White NJ: Melioidosis. Lancet 2003, 361(9370):1715-1722.
• [20]Ngauy V, Lemeshev Y, Sadkowski L, Crawford G: Cutaneous melioidosis in a man who was taken as a prisoner of war by the Japanese during World War II. J Clin Microbiol 2005, 43(2):970-972.
• [21]Bossi P, Tegnell A, Baka A, Van Loock F, Hendriks J, Werner A, Maidhof H, Gouvras G: Bichat guidelines for the clinical management of glanders and melioidosis and bioterrorism-related glanders and melioidosis. Euro Surveill 2004, 9(12):E17-E18.
• [22]Kekulé AS, Bartling C, Beyer W, Bockemühl J, Diller R, Dobler G, Essbauer S, Finke EJ, Fleischer B, Frangoulidis D, Hagen RM, Henning K, Meyer H, Neubauer H, Oehme A, Pauli G, Pfeffer M, Rakin A, Schmitz H, Splettstösser WD, Sprague LD, Tomaso H, Wölfel R, Zimmermann P: Mikrobiologisch-infektiologische Qualitätsstandards (MiQ), Hochpathogene Erreger - Biologische Kampfstoffe, vol. 27. Elsevier, München; 2008.