N-chlorotaurine is highly active against respiratory viruses including SARS-CoV-2 (COVID-19) in vitro

Emerging Microbes and Infections

Jennifer Fenner¹  Angela Luttick¹  Carsten Schwarz²  Paul Tone³  Michaela Lackner⁴  Johannes Ströhle⁴  Markus Nagl⁴  Bettina Sarg⁵  Leopold Kremser⁵  Brigitte Müllauer⁶  Annika Rössler⁶  Zoltan Banki⁶  André Volland⁶  Dorothee von Laer⁶  Heribert Stoiber⁶  Thorsten Wolff⁷  Marlena Nastassja Stadtmüller⁷ 

[1] 360biolabs Pty Ltd, Melbourne, Australia;CF Center Westbrandenburg, Division Cystic Fibrosis, Pediatric Clinic Westbrandenburg, Potsdam, Germany;Innovative Biomedical Concepts, Inc., Staten Island, NY, USA;Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria;Institute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria;Institute of Virology, Medical University of Innsbruck, Innsbruck, Austria;Unit 17-Influenza and Other Respiratory Viruses, Robert Koch-Institute, Berlin, Germany;

关键词: N-chlorotaurine;    COVID-19;    influenza;    respiratory syncytial virus;    antiviral;    anti-infective;   

DOI  :  10.1080/22221751.2022.2065932

来源: DOAJ

【 摘 要 】

N-chlorotaurine (NCT) a long-lived oxidant generated by leukocytes, can be synthesized chemically and applied topically as an anti-infective to different body sites, including the lung via inhalation. Here, we demonstrate the activity of NCT against viruses causing acute respiratory tract infections, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza viruses, and respiratory syncytial virus (RSV). Virucidal activity of NCT was tested in plaque assays, confirmed by RT-qPCR assays. Attack on virus proteins was investigated by mass spectrometry. NCT revealed broad virucidal activity against all viruses tested at 37°C and pH 7. A significant reduction in infectious particles of SARS-CoV-2 isolates from early 2020 by 1 log10 was detected after 15 min of incubation in 1% NCT. Proteinaceous material simulating body fluids enhanced this activity by transchlorination mechanisms (1 −2 log10 reduction within 1–10 min). Tested SARS-CoV-2 variants B.1.1.7 (Alpha) und B.1.351 (Beta) showed a similar susceptibility. Influenza virus infectious particles were reduced by 3 log10 (H3N2) to 5 log10 (H1N1pdm), RSV by 4 log10 within a few min. Mass spectrometry of NCT-treated SARS-CoV-2 spike protein and 3C-like protease, influenza virus haemagglutinin and neuraminidase, and RSV fusion glycoprotein disclosed multiple sites of chlorination and oxidation as the molecular mechanism of action. Application of 1.0% NCT as a prophylactic and therapeutic strategy against acute viral respiratory tract infections deserves comprehensive clinical investigation.

【 授权许可】

Unknown