High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression

Life

Lada A. Ivanova¹  Roman A. Illarionov²  Andrey S. Glotov²  Elena S. Vashukova²  Mariya G. Butenko²  Natalya O. Yurkina²  Olga V. Pachuliia²  Olesya N. Bespalova²  Polina Y. Kozyulina²  Tatyana B. Postnikova²  Marina S. Zainulina³  Dina R. Eremeeva³ 

[1] Antenatal Clinic No.26 Maternity Hospital No.10, 198259 St. Petersburg, Russia;Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia;II Obstetric Department Pathology of Pregnancy, V. F. Snegirev Maternity Hospital No.6, 192014 St. Petersburg, Russia;

关键词: microRNA;    pregnancy;    plasma;    serum;    high-throughput sequencing;   

DOI  :  10.3390/life11101055

来源: DOAJ

【 摘 要 】

Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy (p-value adjusted < 0.05). In addition, we observed differences for 36 miRNAs between plasma and serum (p-value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR (p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities.

【 授权许可】

Unknown