Methodological challenges in utilizing miRNAs as circulating biomarkers

Journal of Cellular and Molecular Medicine

Leni Moldovan²  Kara E. Batte¹  Joanne Trgovcich³  Jon Wisler³  Clay B. Marsh² 

[1]Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH, USA

[2]Division of Pulmonary, Allergy, Critical Care, Sleep Medicine, College of Medicine, The Ohio State University, Columbus, OH, USA

[3]The Department of Surgery, College of Medicine, The Ohio State University, Columbus, OH, USA

关键词: biomarkers;    microRNA;    circulating microRNAs;    plasma;    serum;    real‐time PCR;    profiling;   

DOI  :  10.1111/jcmm.12236

来源: Wiley

【 摘 要 】

Abstract

MicroRNAs (miRNAs) have emerged as important regulators in the post-transcriptional control of gene expression. The discovery of their presence not only in tissues but also in extratissular fluids, including blood, urine and cerebro-spinal fluid, together with their changes in expression in various pathological conditions, has implicated these extracellular miRNAs as informative biomarkers of disease. However, exploiting miRNAs in this capacity requires methodological rigour. Here, we report several key procedural aspects of miRNA isolation from plasma and serum, as exemplified by research in cardiovascular and pulmonary diseases. We also highlight the advantages and disadvantages of various profiling methods to determine the expression levels of plasma- and serum-derived miRNAs. Attention to such methodological details is critical, as circulating miRNAs become diagnostic tools for various human diseases.

【 授权许可】

CC BY   
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.