Protocol for assessing senescence-associated lung pathologies in mice

STAR Protocols

Michihiro Hashimoto¹  Masataka Sugimoto²  Ryuta Mikawa³  Koichiro Kawaguchi⁴  Tadashi Sato⁴  Azusa Asai⁴ 

[1] Corresponding author;Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan;Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa 078-8510, Japan;Research Institute, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan;

关键词: Cell Biology;    Health Sciences;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Cellular senescence underlies tissue aging and aging-associated pathologies, as well as lung pathology. We and others have shown that elimination of senescent cells alleviates pulmonary diseases such as fibrosis and emphysema in animal models. We herein describe a protocol for assessing senescence-dependent lung phenotypes in mice. This protocol describes the use of ARF-DTR mice for semi-genetic elimination of lung senescent cells, followed by a pulmonary function test and the combination with pulmonary disease models to study lung pathologies.For complete details on the use and execution of this protocol, please refer to Hashimoto et al. (2016), Kawaguchi et al. (2021), and Mikawa et al. (2018).

【 授权许可】

Unknown