Agronomy | |
Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions | |
Yawen Zeng1  Muhammad Arslan Iqbal2  Saddam Hussain3  Muhammad Iqbal4  Muhammad Majid Yar4  Humayun Raza4  Hafiz Ghulam Muhu-Din Ahmed4  Aziz Ullah5  Nadeem Sarwar6  Muhammad Nouman Iqbal7  Muhammad Imran8  | |
[1] Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;DHQ Hospital Muzaffargarh, Muzaffargarh 34200, Pakistan;Department of Agronomy, University of Agriculture, Faisalabad 38040, Pakistan;Department of Plant Breeding and Genetics, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan;Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Sargodha, Sargodha 40100, Pakistan;Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad 38000, Pakistan;Rural Health Center Sardar Pur Kabirwala, Khanewal 58150, Pakistan;Soil and Water Testing Laboratory, Khanewal 58150, Pakistan; | |
关键词: genome-wide association study; marker-trait association; water shortage; SNPs; yield; | |
DOI : 10.3390/agronomy11081646 | |
来源: DOAJ |
【 摘 要 】
Genome-wide association study (GWAS) was performed for stomata- and yield-related attributes with high-density Illumina 90 K Infinium SNP (single nucleotide polymorphism) array in bread wheat to determine genetic potential of germplasm for scarce water resources with sustainable yield potential. Major yield and stomata attributes were phenotyped on a panel of Pakistani and foreign accessions grown in non-stressed and water shortage environments during two seasons. Highly significant variations were shown among accessions in both conditions for examined characteristics. Water shortage conditions reduced the overall wheat yield and strong positive correlation existed among stomatal frequency, leaf venation and grain yield per plant. Population structure analyses based on 90,000 SNP data classified the accessions into four sub-populations which indicated the presence of genetic variability. Marker-trait association (MTA) analyses revealed that 422 significant SNPs at p ≤ 10−3, after crossing the false discovery rate (FDR) <0.05 threshold, were linked with examined attributes. Pleiotropic loci (wsnp_Ex_c8913_14881924 and Tdurum_contig10598_304) were associated with flag leaf area (FLA), stomata size (SS), stomata frequency (SF), leaf venation (LV), number of grain per spike (NGS) and grain yield per plant (GYP), which were located on chromosome 4B and 6B at the positions 173.63cM and 229.64cM, respectively, under water shortage conditions. Pleotropic loci wsnp_Ex_c24167_33416760, wsnp_Ex_c5412_9564046 and Tdurum_contig81797_369 on chromosomes 7A, 2A and 4B at the positions 148.26cM, 261.05cM and 173.63cM, respectively, were significantly linked with stomata and yield indices such as FLA, SS, SF, LV, NGS and GYP under normal and water shortage conditions. The current experiment not only validated several MTAs for studied indices reported in other studies but also discovered novel MTAs significant under water shortage environments. Associated and significant SNPs will be useful in discovering novel genes underpinning water shortage tolerance in bread wheat for producing high-yielding and drought tolerant wheat varieties to fulfill the wheat demand for growing populations.
【 授权许可】
Unknown