International Journal of Infectious Diseases | |
Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis | |
Patcharaporn Boonroumkaew1  Somjintana Tourtip1  Lakkhana Sadaow1  Pewpan M. Intapan1  Penchom Janwan2  Wanchai Maleewong2  Oranuch Sanpool2  Rutchanee Rodpai2  Tongjit Thanchomnang3  | |
[1] Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand;Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand; | |
关键词: Trichinellosis; Trichinella; Diagnosis; Immunochromatographic test; Screening test; Rapid test; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Objective: Trichinellosis is a globally distributed food-borne parasitic disease. Initially, diagnosis is usually made based on clinical signs, symptoms, and history of eating raw or undercooked meat. In the present study, an immunochromatographic test (ICT) kit was developed to diagnose trichinellosis by detecting IgG antibodies in sera of infected humans.Methods: Somatic extract from Trichinella spiralis larvae was used as the antigen for the ICT kit development. Diagnostic efficacy was evaluated using human serum samples from proven trichinellosis patients, healthy persons, and those with other parasitic infections.Results: The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy of the ICT kit were 100.0% (95%; CI 88.8 to 100.0%), 92.5% (95% CI; 86.9 to 96.2%), 73.8% (95% CI; 58.0 to 86.1%), 100.0% (95% CI; 97.3 to 100.0%) and 93.8% (95% CI; 89.2 to 96.9%), respectively.Conclusions: This ICT diagnostic kit can be used as a testing tool for human trichinellosis. This test should permit rapid detection of infection to enable prompt anthelminthic treatment. It can also be used for retrospective diagnoses and field surveys based at laboratories where sophisticated equipment is lacking.
【 授权许可】
Unknown