Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions

eLife

Melvin Rodriguez-Heredia¹  Anja Krieger-Liszkay²  Chris Duffy³  Manuel Twachtmann⁴  Guy Thomas Hanke⁵  Robert J Knell⁶  Manuela Kramer⁶  Laura Mosebach⁶  Francesco Saccon⁶  Giovanni Finazzi⁶ 

[1] Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück, Osnabrück, Germany;Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück, Osnabrück, Germany;Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, Paris, France;Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany;Laboratoire de Physiologie Cellulaire et Végétale, UMR 5168, Centre National de la Recherche Scientifique (CNRS), Commissariat a` l’Energie Atomique et aux Energies Alternatives (CEA), Université Grenoble Alpes, Institut National Recherche Agronomique (INRA), Institut de Recherche en Sciences et Technologies pour le Vivant (iRTSV), CEA Grenoble, Grenoble, France;School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom;

关键词: electron transport;    ferredoxin;    thylakoid;    photosynthesis;    chloroplast;    NADPH;   

DOI  :  10.7554/eLife.56088

来源: DOAJ

【 摘 要 】

During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.

【 授权许可】

Unknown