Advances in the Function and Regulation of Hydrogenase in the Cyanobacterium Synechocystis PCC6803

International Journal of Molecular Sciences

Corinne Cassier-Chauvat¹  Théo Veaudor¹  Franck Chauvat² 

[1] UMR8221, CEA, CNRS, University of Paris XI, Institute of Biology and Technology Saclay, Laboratory of Biology and Biotechnology of Cyanobacteria, CEA-Saclay, Gif sur Yvette 91 191, France;

关键词: hydrogen;    bioproduction;    cyanobacteria;    oxidative stress;    regulation;    cysteine oxidation;    sugar stress;    electron transport;    ferredoxin;   

DOI  :  10.3390/ijms151119938

来源: mdpi

【 摘 要 】

In order to use cyanobacteria for the biological production of hydrogen, it is important to thoroughly study the function and the regulation of the hydrogen-production machine in order to better understand its role in the global cell metabolism and identify bottlenecks limiting H₂ production. Most of the recent advances in our understanding of the bidirectional [Ni-Fe] hydrogenase (Hox) came from investigations performed in the widely-used model cyanobacterium Synechocystis PCC6803 where Hox is the sole enzyme capable of combining electrons with protons to produce H₂ under specific conditions. Recent findings suggested that the Hox enzyme can receive electrons from not only NAD(P)H as usually shown, but also, or even preferentially, from ferredoxin. Furthermore, plasmid-encoded functions and glutathionylation (the formation of a mixed-disulfide between the cysteines residues of a protein and the cysteine residue of glutathione) are proposed as possible new players in the function and regulation of hydrogen production.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.