Setting Up an Ultra-Fast Next-Generation Sequencing Approach as Reflex Testing at Diagnosis of Non-Squamous Non-Small Cell Lung Cancer; Experience of a Single Center (LPCE, Nice, France)

Cancers

Maryline Allegra¹  Myriam Salah¹  Virginie Tanga¹  Doriane Bohly¹  Simon Heeke²  Charles-Hugo Marquette³  Jonathan Benzaquen³  Véronique Hofman⁴  Christophe Bontoux⁴  Sandra Lassalle⁴  Virginie Lespinet-Fabre⁴  Elodie Long-Mira⁴  Paul Hofman⁴  Olivier Bordone⁴  Marius Ilié⁴  Salomé Lalvée⁴ 

[1] Biobank-related Hospital (BB-0033-00025), Pasteur Hospital, 06000 Nice, France;Department of Thoracic/Head and Neck Medical Oncology, MD Anderson Cancer Center, Houston, TX 77030, USA;FHU OncoAge, Pasteur Hospital, Université Côte d’Azur, 06000 Nice, France;Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Université Côte d’Azur, 06000 Nice, France;

关键词: genomic alteration;    next-generation sequencing;    turnaround time;    non-squamous non-small cell lung carcinoma;    targeted therapy;   

DOI  :  10.3390/cancers14092258

来源: DOAJ

【 摘 要 】

The number of genomic alterations required for targeted therapy of non-squamous non-small cell lung cancer (NS-NSCLC) patients has increased and become more complex these last few years. These molecular abnormalities lead to treatment that provides improvement in overall survival for certain patients. However, these treated tumors inexorably develop mechanisms of resistance, some of which can be targeted with new therapies. The characterization of the genomic alterations needs to be performed in a short turnaround time (TAT), as indicated by the international guidelines. The origin of the tissue biopsies used for the analyses is diverse, but their size is progressively decreasing due to the development of less invasive methods. In this respect, the pathologists are facing a number of different challenges requiring them to set up efficient molecular technologies while maintaining a strategy that allows rapid diagnosis. We report here our experience concerning the development of an optimal workflow for genomic alteration assessment as reflex testing in routine clinical practice at diagnosis for NS-NSCLC patients by using an ultra-fast-next generation sequencing approach (Ion Torrent Genexus Sequencer, Thermo Fisher Scientific). We show that the molecular targets currently available to personalized medicine in thoracic oncology can be identified using this system in an appropriate TAT, notably when only a small amount of nucleic acids is available. We discuss the new challenges and the perspectives of using such an ultra-fast NGS in daily practice.

【 授权许可】

Unknown