Immature Seed Endosperm and Embryo Proteomics of the Lotus (Nelumbo Nucifera Gaertn.) by One-Dimensional Gel-Based Tandem Mass Spectrometry and a Comparison with the Mature Endosperm Proteome

Proteomes

Junko Shibato¹  Seiji Shioda¹  Randeep Rakwal¹  Masami Yonekura²  Carlo F. Moro²  Yoshiaki Kouzuma²  Yoichiro Fukao³  Ganesh Kumar Agrawal⁴ 

[1] Global Research Center for Innovative Life Sciences, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan;Laboratory of Molecular Food Functionality, College of Agriculture, Ibaraki University, Ami, Ibaraki 300-0393, Japan;Plant Global Educational Project, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan;Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO 13265, Kathmandu 44600, Nepal;

关键词: 1-DGE;    LC-MS/MS;    lotus;    seed;    proteome analysis;    plant proteomics;   

DOI  :  10.3390/proteomes3030184

来源: DOAJ

【 摘 要 】

Lotus (Nelumbo nucifera Gaertn.) seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed proteins and a comparison between the mature and immature endosperm tissues across the seed’s development.One-dimensional gel electrophoresis (SDS-PAGE) linked with tandem mass spectrometry provided a protein inventory of the immature endosperm (122 non-redundant proteins) and embryo (141 non-redundant proteins) tissues. Comparing with the previous mature endosperm dataset (66 non-redundant proteins), a total of 206 non-redundant proteins were identified across all three tissues of the lotus seed. Results revealed some significant differences in proteome composition between the three lotus seed tissues, most notably between the mature endosperm and its immature developmental stage shifting the proteins from nutrient production to nutrient storage.

【 授权许可】

Unknown