Response Surface Methodology Approach for the Synthesis of Ethyl Butyrate

Food Technology and Biotechnology

Carlos André Veiga Burkert¹  Vitor Cardoso Aragão¹  Maria Rita Alaniz Porto¹  Susana Juliano Kalil¹  Janaína Fernandes de Medeiros Burkert¹ 

[1] Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil;

关键词: ethyl butyrate;    immobilized lipase;    esterification;    optimization;    enzymatic synthesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Response surface methodology was used to determine optimum conditions for the esterification of ethanol and butyric acid to produce a flavour ester using immobilized lipase. Various reaction parameters including butyric acid concentration, enzyme concentration, temperature and ethanol/butyric acid molar ratio affecting ethyl butyrate production were investigated using a fractional factorial design 24–1. Based on the results from the first factorial design, all of the variables which were significant in the process were selected to be used in a 24 central composite rotatable design (CCRD). The optimum conditions for the enzymatic reaction were obtained at a 90 mM butyric acid concentration using a 7.7 g/L enzyme concentration at 45 °C and the ethanol/butyric acid molar ratio of 1:1 for 3 h. The esterification percentage, under these conditions, was 87 %.

【 授权许可】

Unknown