Food and Agricultural Immunology | |
The effects of diet and streptozotocin on metabolism and gut microbiota in a type 2 diabetes mellitus mouse model | |
Qun Shen1  Xiaosong Hu1  Ruiyang Yin1  Yong Xue1  Jinrong Hu1  | |
[1] Key Laboratory of Plant Protein and Grain processing, National Engineering Research Center for Fruits and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University; | |
关键词: high-fat diet; high-sucrose-fat diet; diabetes; metabolism; gut microbiota; streptozotocin; | |
DOI : 10.1080/09540105.2020.1761302 | |
来源: DOAJ |
【 摘 要 】
Diabetes mellitus is a metabolic disorder disease. Diabetes models can be established using diet and/or chemical agents. However, the effects of these methods on the intestinal microbiota are poorly understood. In this study, C57BL/6 mice were fed with a high-fat or high-sucrose-fat diet (HFD/HSFD), followed by 1 or 2 injections of streptozotocin (STZ) to induce type 2 diabetes mellitus. HFD-fed mice showed more severe dyslipidemia compared to HSFD-fed mice. The abundance of Bacteroidetes decreased in HFD-fed mice compared to HSFD-fed mice. After STZ injection, all the mice developed more severe hyperglycaemia, particularly in HFD-fed mice. Although the compositions of intestinal microbiota between the diet- and STZ-induced mice were similar, STZ injection increased the abundance of Ruminiclostridium and [Eubacterium] oxidoreducens and decreased the abundance of PrevotellaceaeUCG_001. In summary, HFD and HSFD had different metabolic phenotypes and gut microbiota. Diets play a more vital role than STZ injection in reshaping gut microbiota.
【 授权许可】
Unknown