Genomic Analysis and Delineation of the Tan Spot Susceptibility Locus Tsc1 in Wheat

Frontiers in Plant Science

Arron H. Carter¹  Maricelis Acevedo²  Jason D. Zurn³  Gayan K. Kariyawasam⁴  Zhaohui Liu⁴  Katherine L. D. Running⁵  Justin D. Faris⁶  Aliya Momotaz⁷ 

[1] Department of Crop and Soil Sciences, Washington State University, Pullman, WA, United States;Department of Global Development, Cornell University, Ithaca, NY, United States;Department of Plant Pathology, Kansas State University, Manhattan, KS, United States;Department of Plant Pathology, North Dakota State University, Fargo, ND, United States;Department of Plant Science, North Dakota State University, Fargo, ND, United States;USDA-Agricultural Research Service, Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, Fargo, ND, United States;USDA-Agricultural Research Service, Sugarcane Field Station, Canal Point, FL, United States;

关键词: tan spot;    wheat;    Triticum;    Pyrenophora tritici-repentis;    Ptr ToxC;    Tsc1;   

DOI  :  10.3389/fpls.2022.793925

来源: DOAJ

【 摘 要 】

The necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr) causes the foliar disease tan spot in both bread wheat and durum wheat. Wheat lines carrying the tan spot susceptibility gene Tsc1 are sensitive to the Ptr-produced necrotrophic effector (NE) Ptr ToxC. A compatible interaction results in leaf chlorosis, reducing yield by decreasing the photosynthetic area of leaves. Developing genetically resistant cultivars will effectively reduce disease incidence. Toward that goal, the production of chlorosis in response to inoculation with Ptr ToxC-producing isolates was mapped in two low-resolution biparental populations derived from LMPG-6 × PI 626573 (LP) and Louise × Penawawa (LouPen). In total, 58 genetic markers were developed and mapped, delineating the Tsc1 candidate gene region to a 1.4 centiMorgan (cM) genetic interval spanning 184 kb on the short arm of chromosome 1A. A total of nine candidate genes were identified in the Chinese Spring reference genome, seven with protein domains characteristic of resistance genes. Mapping of the chlorotic phenotype, development of genetic markers, both for genetic mapping and marker-assisted selection (MAS), and the identification of Tsc1 candidate genes provide a foundation for map-based cloning of Tsc1.

【 授权许可】

Unknown