A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?

Frontiers in Bioengineering and Biotechnology

Yong Huang¹  Bing Ren¹  Daniela Lucas²  Arinola O. Lampejo²  Walter L. Murfee²  Carmen C. Dominguez²  Banks M. Lomel²  Christian M. Nguyen²  Nien-Wen Hu² 

[1] Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States;J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States;

关键词: angiogenesis;    lymphangiogenesis;    microcirculation;    tissue engeneering;    biomimetic;   

DOI  :  10.3389/fbioe.2022.912073

来源: DOAJ

【 摘 要 】

The gap between in vitro and in vivo assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed in vivo. A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between in vitro and in vivo assays and guide how vascular biologists and physiologists might think about the microcirculation.

【 授权许可】

Unknown